Emily Hodges
Faculty Member
Last active: 4/26/2017

Directional DNA methylation changes and complex intermediate states accompany lineage specificity in the adult hematopoietic compartment.

Hodges E, Molaro A, Dos Santos CO, Thekkat P, Song Q, Uren PJ, Park J, Butler J, Rafii S, McCombie WR, Smith AD, Hannon GJ
Mol Cell. 2011 44 (1): 17-28

PMID: 21924933 · PMCID: PMC3412369 · DOI:10.1016/j.molcel.2011.08.026

DNA methylation has been implicated as an epigenetic component of mechanisms that stabilize cell-fate decisions. Here, we have characterized the methylomes of human female hematopoietic stem/progenitor cells (HSPCs) and mature cells from the myeloid and lymphoid lineages. Hypomethylated regions (HMRs) associated with lineage-specific genes were often methylated in the opposing lineage. In HSPCs, these sites tended to show intermediate, complex patterns that resolve to uniformity upon differentiation, by increased or decreased methylation. Promoter HMRs shared across diverse cell types typically display a constitutive core that expands and contracts in a lineage-specific manner to fine-tune the expression of associated genes. Many newly identified intergenic HMRs, both constitutive and lineage specific, were enriched for factor binding sites with an implied role in genome organization and regulation of gene expression, respectively. Overall, our studies represent an important reference data set and provide insights into directional changes in DNA methylation as cells adopt terminal fates.

Copyright © 2011 Elsevier Inc. All rights reserved.

MeSH Terms (15)

Adult Binding Sites Cell Differentiation Cell Lineage Comparative Genomic Hybridization DNA Methylation Epigenesis, Genetic Female Gene Expression Regulation Genome, Human Hematopoietic Stem Cells Hematopoietic System Humans Models, Biological Promoter Regions, Genetic

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