Ming Jiang
Last active: 7/6/2016

[Study on p53, mdm-2 and p21WAF1 protein expression in ER-positive and ER-negative human breast cancer cell lines and its relation to biological features].

Jiang M, Shao Z, Zhang Y
Zhonghua Bing Li Xue Za Zhi. 1997 26 (6): 327-30

PMID: 10374318

OBJECTIVE - To study p53, mdm-2 and p21WAF1 protein expression levels in ER-positive and ER-negative human breast cancer cell lines and their relations to biological features.

METHODS - Using cell culture, DNA stable transfection and immunohistochemical methods, the protein expression levels of p53, mdm-2 and WAF1 genes in ER-positive expressing wtp53 MCF-7 cells, ER-negative expressing mtp53 MDA-MB-231 cells and ER-transfected MDA-MB-231 cells were determined, and then, their relation to biological features were compared.

RESULTS - (1) The function and expression of p53 protein of MCF-7 and MDA-MB-231 cells were obviously different. The expression levels of mdm-2 and p21WAF1 proteins in MCF-7 cells were higher than those of MDA-MB-231 cells (P < 0.05), The biological features of the former were more favorable than those of the latter. (2) The ER-transfected MDA-MB-231 cells showed lower expression of mtp53 and higher expression of mdm-2 protein (P < 0.05), but no significant difference from that of p21WAF1 protein (P > 0.05). Meanwhile, biological features leading to favorable prognosis were manifested.

CONCLUSION - The ER status of breast cancer cell lines is related to the expression level of p53 and mdm-2 proteins and the biological features.

MeSH Terms (11)

Breast Neoplasms Cyclin-Dependent Kinase Inhibitor p21 Cyclins Humans Neoplasm Proteins Nuclear Proteins Proto-Oncogene Proteins Proto-Oncogene Proteins c-mdm2 Receptors, Estrogen Tumor Cells, Cultured Tumor Suppressor Protein p53

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