Joel Harp
Last active: 7/24/2015

Macromolecular crystal annealing: evaluation of techniques and variables.

Harp JM, Hanson BL, Timm DE, Bunick GJ
Acta Crystallogr D Biol Crystallogr. 1999 55 (Pt 7): 1329-34

PMID: 10393299 · DOI:10.1107/s0907444999005442

Additional examples of successful application of macromolecular crystal annealing are presented. A qualitative evaluation of variables related to the annealing process was conducted using a variety of macromolecular crystals to determine in which cases parameters may be varied and in which cases the original macromolecular crystal annealing protocol is preferred. A hypothesis is presented relating the solvent content of the crystal to the specific protocol necessary for the successful application of annealing.

MeSH Terms (3)

Animals Evaluation Studies as Topic Proteins

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