John York
Last active: 3/30/2020

Metabolic Labeling of Inositol Phosphates and Phosphatidylinositols in Yeast and Mammalian Cells.

Hale AT, Clarke BP, York JD
Methods Mol Biol. 2020 2091: 83-92

PMID: 31773572 · PMCID: PMC7293352 · DOI:10.1007/978-1-0716-0167-9_7

Inositol phosphate (IP) and phosphatidylinositol (PI) signaling are critical signal transduction pathways responsible for generating numerous receptor-mediated cellular responses. Biochemical and genetic studies have revealed diverse roles of IP and PI signaling in eukaryotic signaling, but detailed characterization of unique IP and PI signaling profiles in response to different agonists and among cell types remains largely unexplored. Here, we outline steady-state inositol metabolic-labeling techniques that can be leveraged to assess the IP and PI signaling state in eukaryotic cells. This flexible technique can be amended and optimized to your cell line of interest, perturbed with biochemical, genetic, or pharmacological alteration, and used to provide comprehensive inositol profiling in various cellular systems.

MeSH Terms (12)

Animals Biochemical Phenomena Cell Line Chlorocebus aethiops COS Cells HeLa Cells HT29 Cells Humans Inositol Phosphates Phosphatidylinositols Saccharomyces cerevisiae Signal Transduction

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