Carlos Grijalva
Last active: 7/27/2018

A novel real-time RT-PCR assay for influenza C tested in Peruvian children.

Howard LM, Johnson M, Gil AI, Pekosz A, Griffin MR, Edwards KM, Lanata CF, Grijalva CG, Williams JV, RESPIRA-PERU Group
J Clin Virol. 2017 96: 12-16

PMID: 28917132 · PMCID: PMC5901714 · DOI:10.1016/j.jcv.2017.08.014

BACKGROUND - Influenza C virus (ICV) is associated with acute respiratory illness. Yet ICV remains under recognized, with most previous studies using only culture to identify cases.

OBJECTIVES - To develop a sensitive and specific real-time RT-PCR assay for ICV that allows for rapid and accurate detection in a clinical or research setting.

STUDY DESIGN - Multiple ICV sequences obtained from GenBank were analyzed, including 141 hemagglutinin-esterase (HE), 106 matrix (M), and 97 nucleoprotein (NP) sequences. Primers and probes were designed based on conserved regions. Multiple primer-probe sets were tested against multiple ICV strains.

RESULTS - The ICV M and NP genes offered the most conserved sequence regions. Primers and probes based on newer sequence data offered enhanced detection of ICV, especially for low titer specimens. An NP-targeted assay yielded the best performance and was capable of detecting 10-100 RNA copies per reaction. The NP assay detected multiple clinical isolates of ICV collected in a field epidemiology study conducted in Peru.

CONCLUSIONS - We report a new real-time RT-PCR assay for ICV with high sensitivity and specificity.

Copyright © 2017 Elsevier B.V. All rights reserved.

MeSH Terms (12)

Child, Preschool Female Humans Infant Influenza, Human Influenzavirus C Male Molecular Diagnostic Techniques Peru Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Sensitivity and Specificity

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