Mark Denison
Last active: 2/22/2016

Expression and processing of nonstructural proteins of the human astroviruses.

Gibson CA, Chen J, Monroe SA, Denison MR
Adv Exp Med Biol. 1998 440: 387-91

PMID: 9782307 · DOI:10.1007/978-1-4615-5331-1_50

The human astroviruses (HAst) are increasingly recognized as an important cause of gastroenteritis. These viruses contain a 6.8-kb positive-sense, single-stranded RNA molecule that is infectious when transfected into permissive cells. The HAst gene 1 is composed of two open reading frames (ORFs 1a and 1b) connected by a ribosomal frameshift. Gene 1 is predicted to encode two nonstructural polyproteins (pp 1a and pp 1ab), and analysis of the HAst gene 1 sequence has resulted in predictions of a serine proteinase within the ORF1a polyprotein. However, none of the gene 1 proteins have been identified. To examine the expression and processing of the HAst2 gene 1 polyprotein, we have translated pp 1a and pp 1ab in vitro. These ongoing studies will provide the foundation for correlating gene 1 expression in vitro with proteins expressed in virus-infected cells.

MeSH Terms (8)

Antibodies, Viral Gene Expression Genes, Viral Humans Mamastrovirus Peptides Protein Processing, Post-Translational Viral Nonstructural Proteins

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