Mark Denison
Last active: 2/22/2016

Exchange of the coronavirus replicase polyprotein cleavage sites alters protease specificity and processing.

Gadlage MJ, Denison MR
J Virol. 2010 84 (13): 6894-8

PMID: 20427532 · PMCID: PMC2903247 · DOI:10.1128/JVI.00752-10

Coronavirus nonstructural proteins 1 to 3 are processed by one or two papain-like proteases (PLP1 and PLP2) at specific cleavage sites (CS1 to -3). Murine hepatitis virus (MHV) PLP2 and orthologs recognize and cleave at a position following a p4-Leu-X-Gly-Gly-p1 tetrapeptide, but it is unknown whether these residues are sufficient to result in processing by PLP2 at sites normally cleaved by PLP1. We demonstrate that exchange of CS1 and/or CS2 with the CS3 p4-p1 amino acids in engineered MHV mutants switches specificity from PLP1 to PLP2 at CS2, but not at CS1, and results in altered protein processing and virus replication. Thus, the p4-p1 residues are necessary for PLP2 processing but require a specific protein or cleavage site context for optimal PLP recognition and cleavage.

MeSH Terms (8)

Murine hepatitis virus Peptide Hydrolases Polyproteins Protein Processing, Post-Translational RNA Replicase Substrate Specificity Viral Proteins Virus Replication

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