The effects of cholera toxin on cellular energy metabolism.

Snider RM, McKenzie JR, Kraft L, Kozlov E, Wikswo JP, Cliffel DE
Toxins (Basel). 2010 2 (4): 632-48

PMID: 22069603 · PMCID: PMC3153216 · DOI:10.3390/toxins2040632

Multianalyte microphysiometry, a real-time instrument for simultaneous measurement of metabolic analytes in a microfluidic environment, was used to explore the effects of cholera toxin (CTx). Upon exposure of CTx to PC-12 cells, anaerobic respiration was triggered, measured as increases in acid and lactate production and a decrease in the oxygen uptake. We believe the responses observed are due to a CTx-induced activation of adenylate cyclase, increasing cAMP production and resulting in a switch to anaerobic respiration. Inhibitors (H-89, brefeldin A) and stimulators (forskolin) of cAMP were employed to modulate the CTx-induced cAMP responses. The results of this study show the utility of multianalyte microphysiometry to quantitatively determine the dynamic metabolic effects of toxins and affected pathways.

MeSH Terms (9)

Animals Cholera Toxin Cyclic AMP Energy Metabolism Lactic Acid Microfluidic Analytical Techniques Oxygen Consumption PC12 Cells Rats

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