Design and synthesis of an antigenic mimic of the Ebola glycoprotein.

Rutledge RD, Huffman BJ, Cliffel DE, Wright DW
J Mater Res. 2008 23 (12): 3161-3168

PMID: 19609372 · PMCID: PMC2711029 · DOI:10.1557/JMR.2008.0384

An antigenic mimic of the Ebola glycoprotein was synthesized and tested for its ability to be recognized by an anti-Ebola glycoprotein antibody. Epitope-mapping procedures yielded a suitable epitope that, when presented on the surface of a nanoparticle, forms a structure that is recognized by an antibody specific for the native protein. This mimic-antibody interaction has been quantitated through ELISA and QCM-based methods and yielded an affinity (K(d) = 12 × 10(-6) M) within two orders of magnitude of the reported affinity of the native Ebola glycoprotein for the same antibody. These results suggest that the rational design approach described herein is a suitable method for the further development of protein-based antigenic mimics with potential applications in vaccine development and sensor technology.

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