M. Neely
Last active: 8/18/2016

Cortical regulation of striatal medium spiny neuron dendritic remodeling in parkinsonism: modulation of glutamate release reverses dopamine depletion-induced dendritic spine loss.

Garcia BG, Neely MD, Deutch AY
Cereb Cortex. 2010 20 (10): 2423-32

PMID: 20118184 · PMCID: PMC2936803 · DOI:10.1093/cercor/bhp317

Striatal medium spiny neurons (MSNs) receive glutamatergic afferents from the cerebral cortex and dopaminergic inputs from the substantia nigra (SN). Striatal dopamine loss decreases the number of MSN dendritic spines. This loss of spines has been suggested to reflect the removal of tonic dopamine inhibitory control over corticostriatal glutamatergic drive, with increased glutamate release culminating in MSN spine loss. We tested this hypothesis in two ways. We first determined in vivo if decortication reverses or prevents dopamine depletion-induced spine loss by placing motor cortex lesions 4 weeks after, or at the time of, 6-hydroxydopamine lesions of the SN. Animals were sacrificed 4 weeks after cortical lesions. Motor cortex lesions significantly reversed the loss of MSN spines elicited by dopamine denervation; a similar effect was observed in the prevention experiment. We then determined if modulating glutamate release in organotypic cocultures prevented spine loss. Treatment of the cultures with the mGluR2/3 agonist LY379268 to suppress corticostriatal glutamate release completely blocked spine loss in dopamine-denervated cultures. These studies provide the first evidence to show that MSN spine loss associated with parkinsonism can be reversed and point to suppression of corticostriatal glutamate release as a means of slowing progression in Parkinson's disease.

MeSH Terms (20)

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine Analysis of Variance Animals Cerebral Cortex Corpus Striatum Dendrites Disease Models, Animal Dopamine Excitatory Amino Acid Agents Fluoresceins Glutamic Acid Male Neurons Organ Culture Techniques Organic Chemicals Oxidopamine Parkinsonian Disorders Rats Rats, Sprague-Dawley Silver Staining

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