NOTICE -- Labnodes will be unavailable On Saturday evening to Sunday morning for scheduled maintenance. We appreciate your patience and we ask that you let us know if you experience any issues.
NOTICE: Labnodes now supports vumc.org email addresses. If your email changed, please update your profile today.
Jeff Reese
Last active: 5/30/2018

In vivo Raman spectral analysis of impaired cervical remodeling in a mouse model of delayed parturition.

O'Brien CM, Herington JL, Brown N, Pence IJ, Paria BC, Slaughter JC, Reese J, Mahadevan-Jansen A
Sci Rep. 2017 7 (1): 6835

PMID: 28754971 · PMCID: PMC5533720 · DOI:10.1038/s41598-017-07047-5

Monitoring cervical structure and composition during pregnancy has high potential for prediction of preterm birth (PTB), a problem affecting 15 million newborns annually. We use in vivo Raman spectroscopy, a label-free, light-based method that provides a molecular fingerprint to non-invasively investigate normal and impaired cervical remodeling. Prostaglandins stimulate uterine contractions and are clinically used for cervical ripening during pregnancy. Deletion of cyclooxygenase-1 (Cox-1), an enzyme involved in production of these prostaglandins, results in delayed parturition in mice. Contrary to expectation, Cox-1 null mice displayed normal uterine contractility; therefore, this study sought to determine whether cervical changes could explain the parturition differences in Cox-1 null mice and gestation-matched wild type (WT) controls. Raman spectral changes related to extracellular matrix proteins, lipids, and nucleic acids were tracked over pregnancy and found to be significantly delayed in Cox-1 null mice at term. A cervical basis for the parturition delay was confirmed by other ex vivo tests including decreased tissue distensibility, hydration, and elevated progesterone levels in the Cox-1 null mice at term. In conclusion, in vivo Raman spectroscopy non-invasively detected abnormal remodeling in the Cox-1 null mouse, and clearly demonstrated that the cervix plays a key role in their delayed parturition.

MeSH Terms (0)

Connections (2)

This publication is referenced by other Labnodes entities:

Links