Luminescence as a continuous real-time reporter of promoter activity in yeast undergoing respiratory oscillations or cell division rhythms.

Robertson JB, Johnson CH
Methods Mol Biol. 2011 734: 63-79

PMID: 21468985 · PMCID: PMC3433746 · DOI:10.1007/978-1-61779-086-7_4

This chapter describes a method for generating yeast respiratory oscillations in continuous culture and monitoring rhythmic promoter activity of the culture by automated real-time recording of luminescence. These techniques chiefly require the use of a strain of Saccharomyces cerevisiae that has been genetically modified to express firefly luciferase under the control of a promoter of interest and a continuous culture bioreactor that incorporates a photomultiplier apparatus for detecting light emission. Additionally, this chapter describes a method for observing rhythmic (cell cycle-related) promoter activity in small batch cultures of yeast through luminescence monitoring.

MeSH Terms (10)

Aerobiosis Bioreactors Cell Culture Techniques Cell Division Cells, Cultured Genes, Reporter Luminescence Promoter Regions, Genetic Saccharomyces cerevisiae Time Factors

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