Quantification of F2-isoprostanes as a biomarker of oxidative stress.

Milne GL, Sanchez SC, Musiek ES, Morrow JD
Nat Protoc. 2007 2 (1): 221-6

PMID: 17401357 · DOI:10.1038/nprot.2006.375

Oxidant stress has been implicated in a wide variety of disease processes. One method to quantify oxidative injury is to measure lipid peroxidation. Quantification of a group of prostaglandin F(2alpha)-like compounds derived from the nonezymatic oxidation of arachidonic acid, termed the F2-isoprostanes (F2-IsoPs), provides an accurate assessment of oxidative stress both in vitro and in vivo. In fact, in a recent independent study sponsored by the National Institutes of Health (NIH), F2-IsoPs were shown to be the most reliable index of in vivo oxidant stress when compared against other well known biomarkers. This protocol details our laboratory's method to quantify F2-IsoPs in biological fluids and tissues using gas chromatography-mass spectrometry (GC-MS). This procedure can be completed for 12-15 samples in 6-8 h.

MeSH Terms (6)

Biomarkers Chemistry Techniques, Analytical F2-Isoprostanes Gas Chromatography-Mass Spectrometry Humans Oxidative Stress

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