John Wikswo
Faculty Member
Last active: 2/19/2015

Mirrored pyramidal wells for simultaneous multiple vantage point microscopy.

Seale KT, Reiserer RS, Markov DA, Ges IA, Wright C, Janetopoulos C, Wikswo JP
J Microsc. 2008 232 (1): 1-6

PMID: 19017196 · PMCID: PMC3789065 · DOI:10.1111/j.1365-2818.2008.02110.x

We report a novel method for obtaining simultaneous images from multiple vantage points of a microscopic specimen using size-matched microscopic mirrors created from anisotropically etched silicon. The resulting pyramidal wells enable bright-field and fluorescent side-view images, and when combined with z-sectioning, provide additional information for 3D reconstructions of the specimen. We have demonstrated the 3D localization and tracking over time of the centrosome of a live Dictyostelium discoideum. The simultaneous acquisition of images from multiple perspectives also provides a five-fold increase in the theoretical collection efficiency of emitted photons, a property which may be useful for low-light imaging modalities such as bioluminescence, or low abundance surface-marker labelling.

MeSH Terms (7)

Animals Dictyostelium Imaging, Three-Dimensional Microscopy Microscopy, Fluorescence Organelles Specimen Handling

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