Michael Waterman
Faculty Member
Last active: 2/12/2015

Adrenaline stimulates cholesterol side-chain cleavage cytochrome P450 mRNA accumulation in bovine adrenocortical cells.

Ehrhart-Bornstein M, Bornstein SR, Trzeclak WH, Usadel H, Güse-Behling H, Waterman MR, Scherbaum WA
J Endocrinol. 1991 131 (2): R5-8

PMID: 1660516 · DOI:10.1677/joe.0.131r005

The effect of adrenaline on the accumulation of mRNA encoding cholesterol side-chain cleavage cytochrome P450 (P450scc) and cortisol secretion was studied in bovine adrenocortical cells in primary culture. Treatment of cultured cells with adrenaline resulted in a 2-fold increase in mRNA encoding P-450scc, as revealed by Northern blot analysis. Under these conditions the maximal stimulation with ACTH resulted in a 6-fold accumulation of mRNA encoding P450scc. The effect of adrenaline on the expression of P450scc was abolished by the beta-blocker propranolol, while propranolol had no effect on ACTH-induced P450scc mRNA accumulation. Adrenaline stimulated the secretion of cortisol in a dose-dependent manner with a median effective dose of 0.5 mumol/l. The adrenaline-stimulated cortisol secretion amounted to 42% of the effect of ACTH (0.1 nmol/l). Upon adrenaline treatment, cAMP concentration in the culture medium increased about 50-fold over the basal value. It is concluded that the stimulatory action of adrenaline upon cortisol formation requires beta-adrenergic receptors and is due, at least in part, to a cAMP-mediated increases in the accumulation of mRNA encoding P450scc.

MeSH Terms (14)

Adrenal Cortex Adrenocorticotropic Hormone Animals Blotting, Northern Cattle Cells, Cultured Cholesterol Side-Chain Cleavage Enzyme Cyclic AMP Dose-Response Relationship, Drug Epinephrine Hydrocortisone Propranolol RNA, Messenger Stimulation, Chemical

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