Michael Waterman
Faculty Member
Last active: 2/12/2015

Molecular cloning of steroid hydroxylases.

John ME, Simpson ER, Boggaram V, Waterman MR
Endocr Res. 1984 10 (3-4): 319-33

PMID: 6549533 · DOI:10.1080/07435808409036504

Recombinant plasmids specific for bovine adrenocortical cytochromes P-450scc and P-45011 beta have been identified and characterized. Using these cDNA inserts as probes, it is found that tissue specificity of gene expression for these two proteins is as expected. Cytochrome P-450scc mRNA is found in adrenocortical and corpus luteum RNA while cytochrome P-45011 beta mRNA is found only in adrenocortical RNA. Neither mRNA was detected in heart, liver or kidney RNA. Cytochrome P-450scc mRNA is found to be 1.9 kb in length while cytochrome P-45011 beta mRNA is found to be 4 kb in length. Treatment of bovine adrenocortical cells with ACTH or other modulators results in increased levels of cytochrome P-450scc and cytochrome P-45011 beta mRNAs. Cytochrome P-450scc is found to be encoded by a limited number or even a single gene.

MeSH Terms (9)

Adrenal Cortex Animals Cattle Cloning, Molecular Cytochrome P-450 Enzyme System DNA Gene Expression Regulation RNA, Messenger Steroid Hydroxylases

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