Michael Waterman
Faculty Member
Last active: 2/12/2015

Expression of mRNA encoding basic fibroblast growth factor (bFGF) in bovine corpora lutea and cultured luteal cells.

Stirling D, Waterman MR, Simpson ER
J Reprod Fertil. 1991 91 (1): 1-8

PMID: 1847419 · DOI:10.1530/jrf.0.0910001

A radiolabelled cRNA was synthesized using a 1.4 kb cDNA complementary to mRNA encoding bovine basic fibroblast growth factor (bFGF) as a template, and used as a probe to investigate the expression of mRNA encoding bFGF in bovine ovarian tissue, and luteal cells in primary culture. Northern analysis of poly(A +)RNA prepared from follicles and corpora lutea of various stages revealed a major mRNA species of 7 kb in corpora lutea of all stages, the amount of which was higher late in the luteal phase. No hybridizable message was detectable in follicles of any size. When luteal cells were established in primary culture, expression of the 7 kb mRNA species was maintained. This expression was increased markedly when cells were treated with LH/hCG or Bt2cAMP. Prostaglandin F-2 alpha treatment caused a marked decrease in the basal content of this 7 kb mRNA, and also severely impaired the ability of LH to stimulate this expression.

MeSH Terms (15)

Animals Blotting, Northern Bucladesine Cattle Cells, Cultured Chorionic Gonadotropin Corpus Luteum Dinoprost Dose-Response Relationship, Drug Female Fibroblast Growth Factor 2 Luteinizing Hormone Progesterone RNA, Messenger RNA Probes

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