Michael Stone
Faculty Member
Last active: 1/20/2015

NMR analysis of base-pair opening kinetics in DNA.

Szulik MW, Voehler M, Stone MP
Curr Protoc Nucleic Acid Chem. 2014 59: 7.20.1-18

PMID: 25501592 · PMCID: PMC4274747 · DOI:10.1002/0471142700.nc0720s59

Base pairing in nucleic acids plays a crucial role in their structure and function. Differences in the base-pair opening and closing kinetics of individual double-stranded DNA sequences or between chemically modified base pairs provide insight into the recognition of these base pairs by DNA processing enzymes. This unit describes how to quantify the kinetics for localized base pairs by observing changes in the imino proton signals by nuclear magnetic resonance spectroscopy. The determination of all relevant parameters using state-of-the art techniques and NMR instrumentation, including cryoprobes, is discussed.

Copyright © 2014 John Wiley & Sons, Inc.

MeSH Terms (4)

Base Pairing DNA Kinetics Magnetic Resonance Spectroscopy

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