Ned Porter
Faculty Member
Last active: 5/29/2014

Analysis of unsaturated compounds by Ag+ coordination ionspray mass spectrometry: studies of the formation of the Ag+/lipid complex.

Seal JR, Havrilla CM, Porter NA, Hachey DL
J Am Soc Mass Spectrom. 2003 14 (8): 872-80

PMID: 12892911 · DOI:10.1016/S1044-0305(03)00339-8

Coordination ionspray mass spectrometry (CIS-MS) is a useful tool in the detection and identification of cholesterol ester and phospholipid hydroperoxides and diacyl peroxides. Extensive studies of a series of cholesterol esters using CIS-MS revealed the following: (1) Cholesterol esters with equal number of double bonds as the internal standard showed a linear relative response in the mass spectrometer while compounds with non-equal numbers of double bonds gave a nonlinear relative response. (2) Complex adducts containing cholesterol ester, silver ion, AgF, AgBF(4), and 2-propanoxide form when silver is in molar excess of cholesterol esters, reducing the [M + Ag](+) signal. (3) In a mixture of cholesterol esters where silver is limiting, Ch22:6 and Ch20:4 bind to silver at the expense of Ch18:2 and have a higher signal in the mass spectrometer. (4) In a mixture of cholesterol esters where silver concentration is twofold greater than total cholesterol ester concentration, Ch22:6 and Ch20:4 form large complex adducts more frequently than Ch18:2 and have a lower signal in the mass spectrometer.

MeSH Terms (6)

Cations, Monovalent Cholesterol Esters Fats, Unsaturated Lipid Peroxidation Silver Spectrometry, Mass, Electrospray Ionization

Connections (2)

This publication is referenced by other Labnodes entities:

Links