Keith Wilson
Faculty Member
Last active: 11/2/2019

Colon-specific delivery of a probiotic-derived soluble protein ameliorates intestinal inflammation in mice through an EGFR-dependent mechanism.

Yan F, Cao H, Cover TL, Washington MK, Shi Y, Liu L, Chaturvedi R, Peek RM, Wilson KT, Polk DB
J Clin Invest. 2011 121 (6): 2242-53

PMID: 21606592 · PMCID: PMC3104743 · DOI:10.1172/JCI44031

Probiotic bacteria can potentially have beneficial effects on the clinical course of several intestinal disorders, but our understanding of probiotic action is limited. We have identified a probiotic bacteria-derived soluble protein, p40, from Lactobacillus rhamnosus GG (LGG), which prevents cytokine-induced apoptosis in intestinal epithelial cells. In the current study, we analyzed the mechanisms by which p40 regulates cellular responses in intestinal epithelial cells and p40's effects on experimental colitis using mouse models. We show that the recombinant p40 protein activated EGFR, leading to Akt activation. Activation of EGFR by p40 was required for inhibition of cytokine-induced apoptosis in intestinal epithelial cells in vitro and ex vivo. Furthermore, we developed a pectin/zein hydrogel bead system to specifically deliver p40 to the mouse colon, which activated EGFR in colon epithelial cells. Administration of p40-containing beads reduced intestinal epithelial apoptosis and disruption of barrier function in the colon epithelium in an EGFR-dependent manner, thereby preventing and treating DSS-induced intestinal injury and acute colitis. Furthermore, p40 activation of EGFR was required for ameliorating colon epithelial cell apoptosis and chronic inflammation in oxazolone-induced colitis. These data define what we believe to be a previously unrecognized mechanism of probiotic-derived soluble proteins in protecting the intestine from injury and inflammation.

MeSH Terms (24)

Administration, Rectal Animals Apoptosis Bacterial Proteins Colitis Dextran Sulfate Drug Evaluation, Preclinical Enzyme Activation Epithelial Cells ErbB Receptors Hydrogels Intestinal Mucosa Lactobacillus rhamnosus Mice Mice, Inbred C57BL Microspheres Oxazolone Permeability Probiotics Proto-Oncogene Proteins c-akt Quinazolines Recombinant Proteins Signal Transduction Tyrphostins

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