Pierre Massion
Faculty Member
Last active: 1/11/2018

Dimethyl sulfoxide decreases interleukin-8-mediated neutrophil recruitment in the airways.

Massion PP, Lindén A, Inoue H, Mathy M, Grattan KM, Nadel JA
Am J Physiol. 1996 271 (5 Pt 1): L838-43

PMID: 8944728 · DOI:10.1152/ajplung.1996.271.5.L838

In this study, we investigated the role of dimethyl sulfoxide (DMSO) in inhibiting interleukin-8 (IL-8)-mediated neutrophil recruitment induced by Pseudomonas aeruginosa (PA) bacterial supernatant. First, we tested whether DMSO could inhibit IL-8 production induced by PA in human bronchial epithelial (16-HBE) cells in vitro. In these cells, exposure to PA or H2O2 induced IL-8 production dose dependently, an effect that was inhibited by 1% DMSO at both the protein and RNA level. Second, we tested whether DMSO could block the recruitment of neutrophils induced by PA in a bypassed segment of dog trachea in vivo. PA supernatant was placed in the tracheal segment for 6 h in four dogs, and neutrophil recruitment and IL-8 concentrations were measured in the superfusate. DMSO prevented the recruitment of neutrophils and IL-8 production induced by PA time dependently. The results suggest that DMSO may play an anti-inflammatory role in the airway by inhibiting IL-8 production in epithelial cells.

MeSH Terms (17)

Animals Bronchi Cell Line Cells, Cultured Dimethyl Sulfoxide Dogs Enzyme-Linked Immunosorbent Assay Epithelium Gene Expression Humans Hydrogen Peroxide Interleukin-8 Neutrophils Protein Biosynthesis Pseudomonas aeruginosa Trachea Transcription, Genetic

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