Ethan Lee
Faculty Member
Last active: 2/18/2021

High-throughput drug screening of fine-needle aspiration-derived cancer organoids.

Bergdorf K, Phifer C, Bharti V, Westover D, Bauer J, Vilgelm A, Lee E, Weiss V
STAR Protoc. 2020 1 (3): 100212

PMID: 33377106 · PMCID: PMC7757655 · DOI:10.1016/j.xpro.2020.100212

Generation of fine-needle aspiration (FNA)-derived cancer organoids has allowed us to develop a number of downstream applications. In this protocol, we start with organoids cultured in a semi-solid format. We dissociate organoids into single cells and then plate in a 384-well format for high-throughput drug screening. While this method must be fine-tuned for each individual organoid culture, it offers a format well suited for rapidly screening medium-sized drug/compound libraries (500-5,000 molecules) and generating dose-response curves to measure relative efficacy. For complete details on the use and execution of this protocol, please refer to Lee et al. (2020) and Vilgelm et al. (2020).

© 2020 The Authors.

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