Ethan Lee
Faculty Member
Last active: 12/22/2019

Crystallization and preliminary crystallographic studies of Gi alpha 1 and mutants of Gi alpha 1 in the GTP and GDP-bound states.

Coleman DE, Lee E, Mixon MB, Linder ME, Berghuis AM, Gilman AG, Sprang SR
J Mol Biol. 1994 238 (4): 630-4

PMID: 8176751 · DOI:10.1006/jmbi.1994.1320

Several different crystal forms of Gi alpha 1 have been grown and analyzed. Crystals of native protein containing bound GTP gamma S belong to space group P3(1)2(1) or P3(2)2(1) with cell dimensions a,b = 80.6 A and c = 106.3 A and diffract to a resolution of 1.9 A using synchrotron radiation. Crystals of native protein containing bound GDP belong to space group I4 with cell dimensions a,b = 121.3 A, and c = 67.7 A and diffract to 3.0 A. Data sets from crystals grown using mutant proteins have also been obtained and characterized.

MeSH Terms (9)

Crystallization Crystallography, X-Ray GTP-Binding Proteins Guanosine 5'-O-(3-Thiotriphosphate) Guanosine Diphosphate Guanosine Triphosphate Mutation Protein Binding Recombinant Proteins

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