Imaging MS of Rodent Ocular Tissues and the Optic Nerve.

Anderson DMG, Lambert W, Calkins DJ, Ablonczy Z, Crouch RK, Caprioli RM, Schey KL
Methods Mol Biol. 2017 1618: 15-27

PMID: 28523496 · DOI:10.1007/978-1-4939-7051-3_3

The visual system is comprised of many specialized cell types that are essential for relaying sensory information about an animal's surroundings to the brain. The cells present in ocular tissue are notoriously delicate, making it particularly challenging to section thin slices of unfixed tissue. Maintaining the morphology of the native tissue is crucial for accurate observations by either conventional staining techniques or in this instance matrix-assisted laser desorption ionization (MALDI IMS) or imaging using mass spectrometry. As vision loss is a significantly debilitating condition, studying molecular mechanisms involved in the process of vision loss is a critically important area of research.

MeSH Terms (6)

Animals Eye Optic Nerve Rodentia Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Vision Disorders

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