Kelli Boyd
Faculty Member
Last active: 3/20/2014

Characterization of a family of novel cysteine- serine-rich nuclear proteins (CSRNP).

Gingras S, Pelletier S, Boyd K, Ihle JN
PLoS One. 2007 2 (8): e808

PMID: 17726538 · PMCID: PMC1950078 · DOI:10.1371/journal.pone.0000808

Gene array analysis has been widely used to identify genes induced during T cell activation. Our studies identified an immediate early gene that is strongly induced in response to IL-2 in mouse T cells which we named cysteine- serine-rich nuclear protein-1 (CSRNP-1). The human ortholog was previously identified as an AXIN1 induced gene (AXUD1). The protein does not contain sequence defined domains or motifs annotated in public databases, however the gene is a member of a family of three mammalian genes that share conserved regions, including cysteine- and serine-rich regions and a basic domain, they encode nuclear proteins, possess transcriptional activation domain and bind the sequence AGAGTG. Consequently we propose the nomenclature of CSRNP-1, -2 and -3 for the family. To elucidate the physiological functions of CSRNP-1, -2 and -3, we generated mice deficient for each of these genes by homologous recombination in embryonic stem cells. Although the CSRNP proteins have the hallmark of transcription factors and CSRNP-1 expression is highly induced by IL-2, deletion of the individual genes had no obvious consequences on normal mouse development, hematopoiesis or T cell functions. However, combined deficiencies cause partial neonatal lethality suggesting that the genes have redundant functions.

MeSH Terms (12)

Amino Acid Sequence Animals Cells, Cultured Cysteine Humans Interleukin-2 Mice Mice, Transgenic Nuclear Proteins Sequence Alignment Serine Transfection

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