Jennifer Gaddy
Research Instructor
Last active: 9/13/2018

Functional plasticity in the type IV secretion system of Helicobacter pylori.

Barrozo RM, Cooke CL, Hansen LM, Lam AM, Gaddy JA, Johnson EM, Cariaga TA, Suarez G, Peek RM, Cover TL, Solnick JV
PLoS Pathog. 2013 9 (2): e1003189

PMID: 23468628 · PMCID: PMC3585145 · DOI:10.1371/journal.ppat.1003189

Helicobacter pylori causes clinical disease primarily in those individuals infected with a strain that carries the cytotoxin associated gene pathogenicity island (cagPAI). The cagPAI encodes a type IV secretion system (T4SS) that injects the CagA oncoprotein into epithelial cells and is required for induction of the pro-inflammatory cytokine, interleukin-8 (IL-8). CagY is an essential component of the H. pylori T4SS that has an unusual sequence structure, in which an extraordinary number of direct DNA repeats is predicted to cause rearrangements that invariably yield in-frame insertions or deletions. Here we demonstrate in murine and non-human primate models that immune-driven host selection of rearrangements in CagY is sufficient to cause gain or loss of function in the H. pylori T4SS. We propose that CagY functions as a sort of molecular switch or perhaps a rheostat that alters the function of the T4SS and "tunes" the host inflammatory response so as to maximize persistent infection.

MeSH Terms (19)

Animals Antigens, Bacterial Bacterial Proteins Bacterial Secretion Systems DNA, Bacterial Female Helicobacter Infections Helicobacter pylori Host-Pathogen Interactions Interleukin-8 Macaca mulatta Male Mice Mice, Inbred C57BL Mice, Knockout Microscopy, Electron, Scanning Recombination, Genetic Specific Pathogen-Free Organisms Virulence Factors

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