CSF-1 signaling mediates recovery from acute kidney injury.

Zhang MZ, Yao B, Yang S, Jiang L, Wang S, Fan X, Yin H, Wong K, Miyazawa T, Chen J, Chang I, Singh A, Harris RC
J Clin Invest. 2012 122 (12): 4519-32

PMID: 23143303 · PMCID: PMC3533529 · DOI:10.1172/JCI60363

Renal tubule epithelia represent the primary site of damage in acute kidney injury (AKI), a process initiated and propagated by the infiltration of macrophages. Here we investigated the role of resident renal macrophages and dendritic cells in recovery from AKI after ischemia/reperfusion (I/R) injury or a novel diphtheria toxin-induced (DT-induced) model of selective proximal tubule injury in mice. DT-induced AKI was characterized by marked renal proximal tubular cell apoptosis. In both models, macrophage/dendritic cell depletion during the recovery phase increased functional and histologic injury and delayed regeneration. After I/R-induced AKI, there was an early increase in renal macrophages derived from circulating inflammatory (M1) monocytes, followed by accumulation of renal macrophages/dendritic cells with a wound-healing (M2) phenotype. In contrast, DT-induced AKI only generated an increase in M2 cells. In both models, increases in M2 cells resulted largely from in situ proliferation in the kidney. Genetic or pharmacologic inhibition of macrophage colony-stimulating factor (CSF-1) signaling blocked macrophage/dendritic cell proliferation, decreased M2 polarization, and inhibited recovery. These findings demonstrated that CSF-1-mediated expansion and polarization of resident renal macrophages/dendritic cells is an important mechanism mediating renal tubule epithelial regeneration after AKI.

MeSH Terms (21)

Acute Kidney Injury Animals Cell Differentiation Cell Polarity Cell Proliferation Cells, Cultured Dendritic Cells Diphtheria Toxin Kidney Kidney Tubules, Proximal Macrophage Colony-Stimulating Factor Macrophages Male Mice Mice, Inbred C57BL Mice, Transgenic Receptor, Macrophage Colony-Stimulating Factor Recombinant Fusion Proteins Recovery of Function Regeneration Signal Transduction

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