L. Roberts
Last active: 4/6/2017

Even free radicals should follow some rules: a guide to free radical research terminology and methodology.

Forman HJ, Augusto O, Brigelius-Flohe R, Dennery PA, Kalyanaraman B, Ischiropoulos H, Mann GE, Radi R, Roberts LJ, Vina J, Davies KJ
Free Radic Biol Med. 2015 78: 233-5

PMID: 25462642 · DOI:10.1016/j.freeradbiomed.2014.10.504

Free radicals and oxidants are now implicated in physiological responses and in several diseases. Given the wide range of expertise of free radical researchers, application of the greater understanding of chemistry has not been uniformly applied to biological studies. We suggest that some widely used methodologies and terminologies hamper progress and need to be addressed. We make the case for abandonment and judicious use of several methods and terms and suggest practical and viable alternatives. These changes are suggested in four areas: use of fluorescent dyes to identify and quantify reactive species, methods for measurement of lipid peroxidation in complex biological systems, claims of antioxidants as radical scavengers, and use of the terms for reactive species.

Copyright © 2014 Elsevier Inc. All rights reserved.

MeSH Terms (11)

Animals Antioxidants Fluorescent Dyes Free Radicals Free Radical Scavengers Humans Lipid Peroxidation Reactive Nitrogen Species Reactive Oxygen Species Terminology as Topic Thiobarbituric Acid Reactive Substances

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