Anne Kenworthy
Last active: 2/12/2016

Nuclear LC3 Associates with Slowly Diffusing Complexes that Survey the Nucleolus.

Kraft LJ, Manral P, Dowler J, Kenworthy AK
Traffic. 2016 17 (4): 369-99

PMID: 26728248 · PMCID: PMC4975375 · DOI:10.1111/tra.12372

MAP1LC3B (microtubule-associated protein 1 light chain 3, LC3) is a key component of the autophagy pathway, contributing to both cargo selection and autophagosome formation in the cytoplasm. Emerging evidence suggests that nuclear forms of LC3 are also functionally important; however, the mechanisms that facilitate the nuclear targeting and trafficking of LC3 between the nucleus and cytoplasm under steady-state conditions are poorly understood. In this study, we examine how residues known to regulate the interactions between LC3 and other proteins or RNA (F52 L53, R68-R70 and G120) contribute to its nuclear targeting, nucleocytoplasmic transport and association with nucleoli and other nuclear components. We find that residues F52 L53 and R68-70, but not G120, regulate targeting of LC3 to the nucleus, its rates of nucleocytoplasmic transport and the apparent sizes of LC3-associated complexes in the nucleus inferred from fluorescence recovery after photobleaching (FRAP) measurements. We also show that LC3 is enriched in nucleoli and its triple arginine motif is especially important for nucleolar targeting. Finally, we identify a series of candidate nuclear LC3-interacting proteins using mass spectrometry, including MAP1B, tubulin and several 40S ribosomal proteins. These findings suggest LC3 is retained in the nucleus in association with high-molecular weight complexes that continuously scan the nucleolus.

© 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

MeSH Terms (9)

Active Transport, Cell Nucleus Cell Nucleolus HeLa Cells Humans Microtubule-Associated Proteins Protein Binding Ribosomal Proteins RNA Tubulin

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