Simon Hayward
Last active: 2/19/2015

Phenotypic and cytogenetic characterization of a human corpus cavernosum cell line (DS-1).

Dahiya R, Sikka S, Hellstrom WJ, Hayward SW, Narayan P, Tanagho EA, Lue TF
Biochem Mol Biol Int. 1993 30 (3): 559-69

PMID: 8401313

We report here the establishment and characterization of a corpus cavernosum cell line (DS-1) from human penile tissue. This is the first cell line of its type derived from cavernosum tissue. DS-1 cells have become immortalized in culture, and show growth in monolayers. These cells have a doubling time of about 45 h in in vitro culture. Cytogenetic analysis by G-banding demonstrated a diploid karyotype with a model chromosome number of 46. The chromosome constitution of DS-1 cells was found to be male (XY), in 28/30 cells scored. Two of the 30 cells showed an extra structurally rearranged "marker" chromosome, that appeared to be a derivative of chromosome 18 with excessive chromosome on the short arm. Ploidy analysis revealed that the majority of DS-1 cells had a DNA index of one. About 35% cells were found to be in G-1 phase and 52% cells in S phase. Light and electron microscopy of DS-1 cells and original penile tissue showed typical characteristics of this tissue. Immunocytochemistry studies using antibodies to smooth muscle actin, desmin, vimentin and cytokeratin (LP34, CAM5.2) showed that the DS-1 cell line had predominantly smooth muscle cells, as these cells were positive for smooth muscle actin, desmin and vimentin.

MeSH Terms (12)

Cell Division Cell Line Cytoskeletal Proteins Erectile Dysfunction Fluorescent Antibody Technique Humans Karyotyping Male Middle Aged Penis Phenotype Ploidies

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