Simon Hayward
Last active: 2/19/2015

Establishment and characterization of an immortalized but non-transformed human prostate epithelial cell line: BPH-1.

Hayward SW, Dahiya R, Cunha GR, Bartek J, Deshpande N, Narayan P
In Vitro Cell Dev Biol Anim. 1995 31 (1): 14-24

PMID: 7535634 · DOI:10.1007/BF02631333

This report describes the development and characterization of an epithelial cell line (BPH-1) from human prostate tissue obtained by transurethral resection. Primary epithelial cell cultures were immortalized with SV40 large T antigen. One of the isolated clones was designated BPH-1. These cells have a cobblestone appearance in monolayer culture and are non-tumorigenic in nude mice following subcutaneous injection or subrenal capsule grafting. They express the SV40 large T antigen and exhibit increased levels of p53, as determined by immunocytochemistry. Cytogenetic analysis by G-banding demonstrated an aneuploid karyotype with a modal chromosome number of 76 (range 71 to 79, n = 28) and 6 to 8 marker chromosomes. Some structurally rearranged chromosomes were observed, but the Y chromosome was normal. The expressed cytokeratin profile was consistent with a prostatic luminal epithelial cell. This profile was the same as that of primary prostatic epithelial cultures from which the BPH-1 cells were derived. In serum-free culture in plastic dishes epidermal growth factor (EGF), transforming growth factor (TGF)-alpha, fibroblast growth factor (FGF) 1 (aFGF), and FGF 7 (KGF) induced increased proliferation in these cells whereas FGF 2 (bFGF), TGF-beta 1, and TGF-beta 2 inhibited proliferative activity. Testosterone had no direct effect on the proliferative rate of BPH-1 cells. 5 alpha-Reductase, 3 alpha-hydroxysteroid oxidoreductase, and 17 beta-hydroxy-steroid oxidoreductase activities were detected in BPH-1 cells. Expression of androgen receptors and the secretory markers, prostate specific antigen and prostatic acid phosphatase, were not detectable by immunocytochemistry, biochemical assay, or RT-PCR analysis.

MeSH Terms (21)

Aged Aneuploidy Animals Antigens, Polyomavirus Transforming Base Sequence Cell Division Cell Line Epithelial Cells Humans Immunohistochemistry Karyotyping Keratins Male Mice Mice, Nude Molecular Sequence Data Prostate Receptors, Androgen RNA, Messenger Testosterone Tumor Suppressor Protein p53

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