Matthew Wilson
Last active: 3/24/2020

Hydrodynamic Renal Pelvis Injection for Non-viral Expression of Proteins in the Kidney.

Woodard LE, Welch RC, Williams FM, Luo W, Cheng J, Wilson MH
J Vis Exp. 2018 (131)

PMID: 29364221 · PMCID: PMC5907682 · DOI:10.3791/56324

Hydrodynamic injection creates a local, high-pressure environment to transfect various tissues with plasmid DNA and other substances. Hydrodynamic tail vein injection, for example, is a well-established method by which the liver can be transfected. This manuscript describes an application of hydrodynamic principles by injection of the mouse kidney directly with plasmid DNA for kidney-specific gene expression. Mice are anesthetized and the kidney is exposed by a flank incision followed by a fast injection of a plasmid DNA-containing solution directly into the renal pelvis. The needle is kept in place for ten seconds and the incision site is sutured. The following day, live animal imaging, Western blot, or immunohistochemistry may be used to assay gene expression, or other assays suited to the transgene of choice are used for detection of the protein of interest. Published methods to prolong gene expression include transposon-mediated transgene integration and cyclophosphamide treatment to inhibit the immune response to the transgene.

MeSH Terms (12)

Animals DNA Hydrodynamics Injections Kidney Kidney Pelvis Male Mice Plasmids Protein Biosynthesis Transfection Transgenes

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