Leslie Gewin
Faculty Member
Last active: 3/26/2019

Substrate Elasticity Governs Differentiation of Renal Tubule Cells in Prolonged Culture.

Love HD, Ao M, Jorgensen S, Swearingen L, Ferrell N, Evans R, Gewin L, Harris RC, Zent R, Roy S, Fissell WH
Tissue Eng Part A. 2019 25 (13-14): 1013-1022

PMID: 30484388 · PMCID: PMC6648172 · DOI:10.1089/ten.TEA.2018.0182

IMPACT STATEMENT - Successful clinical tissue engineering requires functional fidelity of the cultured cell to its counterpart, but this has been elusive in renal tissue engineering. Typically, renal proximal tubule cells in culture have a flattened morphology and do not express key transporters essential to their function. In this article, we show for the first time that substrate mechanical properties dictate differentiation of cultured renal proximal tubule cells. Remarkably, this effect was only discernable after 4 weeks in culture, longer than usually reported for this cell type. These results demonstrate a new tunable parameter to optimize cell differentiation in renal tissue engineering.

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