Renal localization and regulation of 15-hydroxyprostaglandin dehydrogenase.

Yao B, Xu J, Harris RC, Zhang MZ
Am J Physiol Renal Physiol. 2008 294 (2): F433-9

PMID: 18057186 · DOI:10.1152/ajprenal.00436.2007

Tissue prostaglandin levels are determined by both biosynthesis and catabolism. The current studies report the expression and localization of 15-hydroxyprostaglandin dehydrogenase (15-PGDH), a key enzyme in prostaglandin catabolism in the kidneys. We also investigated potential interactions between 15-PGDH and cyclooxygenase (COX), a key enzyme in prostaglandin biosynthesis. Both 15-PGDH mRNA and protein levels were significantly higher in kidney cortex than in papilla, which is opposite to the expression pattern of COX-2. In situ hybridization indicated that 15-PGDH mRNA was mainly localized to the tubular epithelial cells in kidney cortex and outer medulla but not in the glomerulus or papilla. Dual immunofluorescent staining indicated that 15-PGDH was expressed in the proximal tubule, cortical, and outer medullary thick ascending limb and collecting duct but not in the macula densa or papilla. 15-PGDH levels were significantly lower in a macula densa cell line (MMDD1) than in a proximal tubule cell line. Although a high-salt diet decreased COX-2 expression in macula densa, it increased macula densa 15-PGDH expression in both mouse and rat kidneys. In MMDD1 cells, a COX-2 inhibitor increased 15-PGDH, whereas a COX-1 inhibitor had no effect. Furthermore, intense 15-PGDH immunofluorescent staining was found in both macula densa and glomerulus in COX-2 knockout mice. The intrarenal distribution of 15-PGDH and its interactions with COX-2 suggest that differential regulation of COX-2 and 15-PGDH may play an important role in determining levels of prostaglandins involved in regulation of salt, volume, and blood pressure homeostasis.

MeSH Terms (25)

Animals Cell Line Cyclooxygenase 2 Cyclooxygenase Inhibitors Epithelial Cells Epithelium Gene Expression Regulation, Enzymologic Hydroxyprostaglandin Dehydrogenases In Situ Hybridization Kidney Kidney Cortex Kidney Glomerulus Kidney Medulla Kidney Tubules, Proximal Male Mice Mice, Inbred Strains Mice, Knockout Organic Chemicals Pyrazoles Rats Rats, Sprague-Dawley Sodium Chloride, Dietary Sulfonamides Swine

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