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TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton.

Preston Campbell J, Mulcrone P, Masood SK, Karolak M, Merkel A, Hebron K, Zijlstra A, Sterling J, Elefteriou F
Sci Rep. 2015 5: 12635

PMID: 26271202 · PMCID: PMC4536516 · DOI:10.1038/srep12635

Current methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton, concurrently with host gene expression, using TRIzol-based DNA/RNA extraction and Alu sequence qPCR amplification. This approach enables precise quantification of tumor cells and corresponding host gene expression during metastatic colonization in xenograft models.

MeSH Terms (16)

Alu Elements Animals Biomarkers, Tumor Bone Neoplasms Cell Line, Tumor DNA, Neoplasm Gene Expression Profiling Genetic Markers Guanidines Mice Mice, Nude Phenols Real-Time Polymerase Chain Reaction Reproducibility of Results RNA, Neoplasm Sensitivity and Specificity

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