Borden Lacy
Last active: 3/24/2020

Structure and function of anthrax toxin.

Lacy DB, Collier RJ
Curr Top Microbiol Immunol. 2002 271: 61-85

PMID: 12224524 · DOI:10.1007/978-3-662-05767-4_4

Anthrax toxin is a binary A-B toxin comprised of protective antigen (PA) and two enzymatic moieties, edema factor (EF) and lethal factor (LF). In the presence of a host cell-surface receptor, PA can mediate the delivery of EF and LF from the extracellular milieu into the host cell cytosol to effect toxicity. In this delivery, PA undergoes multiple structural changes--from a monomer to a heptameric prepore to a membrane-spanning heptameric pore. The catalytic factors also undergo dramatic structural changes as they unfold to allow for their translocation across the endosomal membrane and refold to preserve their catalytic activity within the cytosol. In addition to these gross structural changes, the intoxication mechanism depends on the ability of PA to form specific interactions with the host cell receptor, EF, and LF. This chapter presents a review of experiments probing these structural interactions and rearrangements in the hopes of gaining a molecular understanding of toxin action.

MeSH Terms (13)

Animals Antigens, Bacterial Bacillus anthracis Bacterial Toxins Binding Sites Biological Transport Carrier Proteins Cell Membrane Endocytosis Humans Models, Molecular Protein Structure, Tertiary Receptors, Cell Surface

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