Analysis of protein composition using multidimensional chromatography and mass spectrometry.

Link AJ, Jennings JL, Washburn MP
Curr Protoc Protein Sci. 2004 Chapter 23: Unit 23.1

PMID: 18429262 · DOI:10.1002/0471140864.ps2301s34

Multidimensional liquid chromatography of peptides produced by protease digestion of complex protein mixtures followed by tandem mass spectrometry can be coupled with automated database searching to identify large numbers of proteins in complex samples. These methods avoid the limitations of gel electrophoresis and in-gel digestions by directly identifying protein mixtures in solution.

MeSH Terms (6)

Angiotensins Buffers Chromatography Mass Spectrometry Proteins Software

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