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Protein identification at the low femtomole level from silver-stained gels using a new fritless electrospray interface for liquid chromatography-microspray and nanospray mass spectrometry.

Gatlin CL, Kleemann GR, Hays LG, Link AJ, Yates JR
Anal Biochem. 1998 263 (1): 93-101

PMID: 9750149 · DOI:10.1006/abio.1998.2809

Conventional capillary liquid chromatography/mass spectrometry (LC/MS) typically employs low microl/min flow rates with gas/liquid sheath to enhance spray stability. Over the past several years a number of reports have demonstrated success with electrospray (ES) interface designs optimized for submicroliter/min flows which have clear advantages in terms of enhancement of detection limit, lower sample consumption, and ability to accommodate a wider range of buffer conditions. We report here a fritless electrospray interface (FESI) design that is inexpensive and robust and can be operated and adapted to accommodate a variety of applications for submicroliter/min flow rates. The novelty of this interface revolves around the use of a fritless microcapillary column and precolumn application of electrospray voltage at a microtee junction to achieve stable microspray and nanospray flow rates. This sheathless FESI device eliminates postcolumn dead volume since small particles (
Copyright 1998 Academic Press.

MeSH Terms (12)

Amino Acid Sequence Chromatography, Liquid Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Fungal Proteins Mass Spectrometry Molecular Sequence Data Peptides Proteins Saccharomyces cerevisiae Sensitivity and Specificity Silver Staining

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