Direct analysis of protein mixtures by tandem mass spectrometry.

Yates JR, McCormack AL, Schieltz D, Carmack E, Link A
J Protein Chem. 1997 16 (5): 495-7

PMID: 9246634 · DOI:10.1023/a:1026365528484

Methods to identify proteins contained in mixtures are described. The approach uses microcolumn liquid chromatography and automated tandem mass spectrometry in conjunction with protein and nucleotide database searching algorithms. This approach is applied to the identification of proteins obtained by immunoprecipitation reactions, interaction with a GST protein fusion products and interaction with a macromolecular complex.

MeSH Terms (7)

Chromatography, Liquid Databases, Factual Macromolecular Substances Mass Spectrometry Precipitin Tests Proteins Sensitivity and Specificity

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