Purifying protein complexes for mass spectrometry: applications to protein translation.

Link AJ, Fleischer TC, Weaver CM, Gerbasi VR, Jennings JL
Methods. 2005 35 (3): 274-90

PMID: 15722224 · DOI:10.1016/j.ymeth.2004.08.019

Proteins control and mediate most of the biological activities in the cell. In most cases, proteins either interact with regulatory proteins or function in large molecular assemblies to carryout biological processes. Understanding the functions of individual proteins requires the identification of these interacting proteins. With its speed and sensitivity, mass spectrometry has become the dominant method for identifying components of protein complexes. This article reviews and discusses various approaches to purify protein complexes and analyze the proteins using mass spectrometry. As examples, methods to isolate and analyze protein complexes responsible for the translation of messenger RNAs into polypeptides are described.

MeSH Terms (18)

Cell Differentiation Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Epitopes Hydrogen-Ion Concentration Immunoprecipitation Mass Spectrometry Models, Biological Protein Biosynthesis Proteins Proteomics Ribosomes RNA, Messenger Saccharomyces cerevisiae Sensitivity and Specificity Sucrose Temperature Time Factors

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