Joseph Roland
Managing Director
Last active: 10/5/2017

MAGI-2 in prostate cancer: an immunohistochemical study.

Goldstein J, Borowsky AD, Goyal R, Roland JT, Arnold SA, Gellert LL, Clark PE, Hameed O, Giannico GA
Hum Pathol. 2016 52: 83-91

PMID: 26980016 · DOI:10.1016/j.humpath.2016.01.003

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 (MAGI-2) is a scaffolding protein that links cell adhesion molecules, receptors, and signaling molecules to the cytoskeleton and maintains the architecture of cell junctions. MAGI-2 gene rearrangements have recently been described in prostate cancer. We studied the immunohistochemical expression of MAGI-2 protein in prostate tissue. Seventy-eight radical prostatectomies were used to construct 3 tissue microarrays consisting of 512 cores, including benign tissue, benign prostatic hyperplasia, high-grade prostatic intraepithelial neoplasia (HGPIN), and adenocarcinoma, Gleason patterns 3 to 5. Immunohistochemistry for phosphatase and tensin homologue (PTEN) and double-stain MAGI-2/p63 was performed and analyzed by visual and image analysis, the latter as percent of analyzed area (%AREA), and mean optical density multiplied by %AREA (STAIN). By visual and image analysis, MAGI-2 was significantly higher in adenocarcinoma and HGPIN compared with benign (benign versus HGPIN P < .001; benign versus adenocarcinoma, P < .001). HGPIN and adenocarcinoma did not significantly differ by either modality. Using visual intensity to distinguish benign tissue and adenocarcinoma, a receiver operating curve yielded an area under the curve of 0.902. A STAIN threshold of 1470 yielded a sensitivity of 0.66 and specificity of 0.96. There was a significant correlation between PTEN and MAGI-2 staining for normal and benign prostatic hyperplasia, but this was lost in HGPIN and cancer. We conclude that MAGI-2 immunoreactivity is elevated in prostate cancer and HGPIN compared with normal tissue, and suggest that MAGI-2 may contribute to prostate carcinogenesis. This is the first report of MAGI-2 staining by immunohistochemistry in prostate cancer.

Copyright © 2016 Elsevier Inc. All rights reserved.

MeSH Terms (24)

Adenocarcinoma Adult Aged Aged, 80 and over Area Under Curve Biomarkers, Tumor Biopsy Carrier Proteins Humans Immunohistochemistry In Situ Hybridization, Fluorescence Male Middle Aged Neoplasm Grading Predictive Value of Tests Prostatic Hyperplasia Prostatic Intraepithelial Neoplasia Prostatic Neoplasms PTEN Phosphohydrolase ROC Curve Tissue Array Analysis Transcription Factors Tumor Suppressor Proteins Up-Regulation

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