H. Alex Brown
Principle Investigator; Professor of Pharmacology, Chemistry, and Biochemistry; Associate Director, VICB;
Last active: 2/12/2015

Biochemical properties of a P2Y-purinergic receptor.

Harden TK, Boyer JL, Brown HA, Cooper CL, Jeffs RA, Martin MW
Ann N Y Acad Sci. 1990 603: 256-66

PMID: 2291525 · DOI:10.1111/j.1749-6632.1990.tb37677.x

The turkey erythrocyte has substantial value as a model for the study of a receptor that exhibits pharmacological properties very similar to those delineated in mammalian tissues for a P2Y-purinergic receptor. The G protein-dependent coupling of this receptor to phospholipase C can be studied in detail, and the availability of an abundant source of homogeneous cells from which highly purified plasma membranes can be prepared, has led to the development of a radiolabeled, reversibly binding radioligand for a P2Y-purinergic receptor and a photoaffinity covalent radiolabel for this receptor. This source of plasma membranes highly enriched in P2Y-purinergic receptors should also serve as a rich starting material for the eventual purification and structural characterization of this important signaling protein.

MeSH Terms (6)

Adenosine Triphosphate Animals Erythrocytes Kinetics Receptors, Purinergic Turkeys

Connections (1)

This publication is referenced by other Labnodes entities:

Links