H. Alex Brown
Principle Investigator; Professor of Pharmacology, Chemistry, and Biochemistry; Associate Director, VICB;
Last active: 2/12/2015

Biochemical properties of a P2Y-purinergic receptor.

Harden TK, Boyer JL, Brown HA, Cooper CL, Jeffs RA, Martin MW
Ann N Y Acad Sci. 1990 603: 256-66

PMID: 2291525 · DOI:10.1111/j.1749-6632.1990.tb37677.x

The turkey erythrocyte has substantial value as a model for the study of a receptor that exhibits pharmacological properties very similar to those delineated in mammalian tissues for a P2Y-purinergic receptor. The G protein-dependent coupling of this receptor to phospholipase C can be studied in detail, and the availability of an abundant source of homogeneous cells from which highly purified plasma membranes can be prepared, has led to the development of a radiolabeled, reversibly binding radioligand for a P2Y-purinergic receptor and a photoaffinity covalent radiolabel for this receptor. This source of plasma membranes highly enriched in P2Y-purinergic receptors should also serve as a rich starting material for the eventual purification and structural characterization of this important signaling protein.

MeSH Terms (6)

Adenosine Triphosphate Animals Erythrocytes Kinetics Receptors, Purinergic Turkeys

Connections (1)

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