Contrasting effects of exercise and NOS inhibition on tissue-specific fatty acid and glucose uptake in mice.

Rottman JN, Bracy D, Malabanan C, Yue Z, Clanton J, Wasserman DH
Am J Physiol Endocrinol Metab. 2002 283 (1): E116-23

PMID: 12067851 · DOI:10.1152/ajpendo.00545.2001

Isotopic techniques were used to test the hypothesis that exercise and nitric oxide synthase (NOS) inhibition have distinct effects on tissue-specific fatty acid and glucose uptakes in a conscious, chronically catheterized mouse model. Uptakes were measured using the radioactive tracers (125)I-labeled beta-methyl-p-iodophenylpentadecanoic acid (BMIPP) and deoxy-[2-(3)H]glucose (DG) during treadmill exercise with and without inhibition of NOS. [(125)I]BMIPP uptake at rest differed substantially among tissues with the highest levels in heart. With exercise, [(125)I]BMIPP uptake increased in both heart and skeletal muscles. In sedentary mice, NOS inhibition induced by nitro-L-arginine methyl ester (L-NAME) feeding increased heart and soleus [(125)I]BMIPP uptake. In contrast, exercise, but not L-NAME feeding, resulted in increased heart and skeletal muscle [2-(3)H]DG uptake. Significant interactions were not observed in the effects of combined exercise and L-NAME feeding on [(125)I]BMIPP and [2-(3)H]DG uptakes. In the conscious mouse, exercise and NOS inhibition produce distinct patterns of tissue-specific fatty acid and glucose uptake; NOS is not required for important components of exercise-associated metabolic signaling, or other mechanisms compensate for the absence of this regulatory mechanism.

MeSH Terms (16)

Administration, Oral Animals Deoxyglucose Enzyme Inhibitors Fatty Acids Glucose Iodine Radioisotopes Iodobenzenes Mice Mice, Inbred C57BL Models, Animal NG-Nitroarginine Methyl Ester Nitric Oxide Synthase Organ Specificity Physical Exertion Tritium

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