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Fiona Yull

Last active: 3/31/2020

NF-kappaB mediates FGF signal regulation of msx-1 expression.

Bushdid PB, Chen CL, Brantley DM, Yull F, Raghow R, Kerr LD, Barnett JV
Dev Biol. 2001 237 (1): 107-15

PMID: 11518509 · DOI:10.1006/dbio.2001.0356

The nuclear factor-kappaB (NF-kappaB) family of transcription factors is involved in proliferation, differentiation, and apoptosis in a stage- and cell-dependent manner. Recent evidence has shown that NF-kappaB activity is necessary for both chicken and mouse limb development. We report here that the NF-kappaB family member c-rel and the homeodomain gene msx-1 have partially overlapping expression patterns in the developing chick limb. In addition, inhibition of NF-kappaB activity resulted in a decrease in msx-1 mRNA expression. Sequence analysis of the msx-1 promoter revealed three potential kappaB-binding sites similar to the interferon-gamma (IFN-gamma) kappaB-binding site. These sites bound to c-Rel, as shown by electrophoretic mobility shift assay (EMSA). Furthermore, inhibition of NF-kappaB activity significantly reduced transactivation of the msx-1 promoter in response to FGF-2/-4, known stimulators of msx-1 expression. These results suggest that NF-kappaB mediates the FGF-2/-4 signal regulation of msx-1 gene expression.

Copyright 2001 Academic Press.

MeSH Terms (13)

Animals Binding Sites Chick Embryo Extremities Fibroblast Growth Factors Gene Expression Regulation, Developmental Homeodomain Proteins MSX1 Transcription Factor NF-kappa B Proto-Oncogene Proteins c-rel RNA, Messenger Transcriptional Activation Transcription Factors

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