Using retroviruses as a mutagenesis tool to explore the zebrafish genome.

Jao LE, Maddison L, Chen W, Burgess SM
Brief Funct Genomic Proteomic. 2008 7 (6): 427-43

PMID: 18977782 · PMCID: PMC2722255 · DOI:10.1093/bfgp/eln038

We review different uses of the retroviral mutagenesis technology as the tool to manipulate the zebrafish genome. In addition to serving as a mutagen in a phenotype-driven forward mutagenesis screen as it was originally adapted for, retroviral insertional mutagenesis can also be exploited in reverse genetic approaches, delivering enhancer- and gene-trap vectors for the purpose of examining gene expression patterns and mutagenesis, making sensitized mutants amenable for chemical and genetic modifier screens, and producing gain-of-function mutations by epigenetically overexpressing the retroviral-inserted genes. From a technology point of view, we also summarize the recent advances in the high-throughput cloning of retroviral integration sites, a pivotal step toward identifying mutations. Lastly, we point to some potential directions that retroviral mutagenesis might take from the lessons of studying other model organisms.

MeSH Terms (6)

Animals Genome Models, Biological Mutagenesis Retroviridae Zebrafish

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