James Crowe
Faculty Member
Last active: 3/31/2020

Development of Human Monoclonal Antibodies Against Respiratory Syncytial Virus Using a High Efficiency Human Hybridoma Technique.

Alvarado G, Crowe JE
Methods Mol Biol. 2016 1442: 63-76

PMID: 27464688 · DOI:10.1007/978-1-4939-3687-8_6

Human monoclonal antibodies against RSV have high potential for use as prophylaxis or therapeutic molecules, and they also can be used to define the structure of protective epitopes for rational vaccine design. In the past, however, isolation of human monoclonal antibodies was difficult and inefficient. Here, we describe contemporary methods for activation and proliferation of primary human memory B cells followed by cytofusion to non-secreting myeloma cells by dielectrophoresis to generate human hybridomas secreting RSV-specific monoclonal antibodies. We also provide experimental methods for screening human B cell lines to obtain RSV-specific lines, especially lines secreting neutralizing antibodies.

MeSH Terms (10)

Antibodies, Monoclonal Antibodies, Neutralizing B-Lymphocytes Cell Line Cell Proliferation Humans Hybridomas Lymphocyte Activation Neutralization Tests Respiratory Syncytial Virus, Human

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