James Crowe
Faculty Member
Last active: 3/31/2020

Frequent Use of the IgA Isotype in Human B Cells Encoding Potent Norovirus-Specific Monoclonal Antibodies That Block HBGA Binding.

Sapparapu G, Czakó R, Alvarado G, Shanker S, Prasad BV, Atmar RL, Estes MK, Crowe JE
PLoS Pathog. 2016 12 (6): e1005719

PMID: 27355511 · PMCID: PMC4927092 · DOI:10.1371/journal.ppat.1005719

Noroviruses (NoV) are the most common cause of non-bacterial acute gastroenteritis and cause local outbreaks of illness, especially in confined situations. Despite being identified four decades ago, the correlates of protection against norovirus gastroenteritis are still being elucidated. Recent studies have shown an association of protection with NoV-specific serum histo-blood group antigen-blocking antibody and with serum IgA in patients vaccinated with NoV VLPs. Here, we describe the isolation and characterization of human monoclonal IgG and IgA antibodies against a GI.I NoV, Norwalk virus (NV). A higher proportion of the IgA antibodies blocked NV VLP binding to glycans than did IgG antibodies. We generated isotype-switched variants of IgG and IgA antibodies to study the effects of the constant domain on blocking and binding activities. The IgA form of antibodies appears to be more potent than the IgG form in blocking norovirus binding to histo-blood group antigens. These studies suggest a unique role for IgA antibodies in protection from NoV infections by blocking attachment to cell receptors.

MeSH Terms (14)

Antibodies, Monoclonal B-Lymphocytes Blood Group Antigens Blotting, Western Caliciviridae Infections Cell Line Enzyme-Linked Immunosorbent Assay Gastroenteritis Humans Hybridomas Immunoglobulin A Immunoglobulin G Norwalk virus Polymerase Chain Reaction

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