James Crowe
Faculty Member
Last active: 3/31/2020

Tissue-specific expressed antibody variable gene repertoires.

Briney BS, Willis JR, Finn JA, McKinney BA, Crowe JE
PLoS One. 2014 9 (6): e100839

PMID: 24956460 · PMCID: PMC4067404 · DOI:10.1371/journal.pone.0100839

Recent developments in genetic technologies allow deep analysis of the sequence diversity of immune repertoires, but little work has been reported on the architecture of immune repertoires in mucosal tissues. Antibodies are the key to prevention of infections at the mucosal surface, but it is currently unclear whether the B cell repertoire at mucosal surfaces reflects the dominant antibodies found in the systemic compartment or whether mucosal tissues harbor unique repertoires. We examined the expressed antibody variable gene repertoires from 10 different human tissues using RNA samples derived from a large number of individuals. The results revealed that mucosal tissues such as stomach, intestine and lung possess unique antibody gene repertoires that differed substantially from those found in lymphoid tissues or peripheral blood. Mutation frequency analysis of mucosal tissue repertoires revealed that they were highly mutated, with little evidence for the presence of naïve B cells, in contrast to blood. Mucosal tissue repertoires possessed longer heavy chain complementarity determining region 3 loops than lymphoid tissue repertoires. We also noted a large increase in frequency of both insertions and deletions in the small intestine antibody repertoire. These data suggest that mucosal immune repertoires are distinct in many ways from the systemic compartment.

MeSH Terms (23)

Adolescent Adult Antibodies Bone Marrow Cluster Analysis Complementarity Determining Regions Demography DNA Gene Expression Regulation Germ Cells High-Throughput Nucleotide Sequencing Humans Immunoglobulin Variable Region Lymphoid Tissue Middle Aged Mucous Membrane Mutation Mutation Rate Organ Specificity RNA Somatic Hypermutation, Immunoglobulin V(D)J Recombination Young Adult

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