A role for the inositol kinase Ipk1 in ciliary beating and length maintenance.

Sarmah B, Winfrey VP, Olson GE, Appel B, Wente SR
Proc Natl Acad Sci U S A. 2007 104 (50): 19843-8

PMID: 18056639 · PMCID: PMC2148385 · DOI:10.1073/pnas.0706934104

Cilia project from cells as membranous extensions, with microtubule structural cores assembling from basal bodies by intraflagellar transport (IFT). Here, we report a ciliary role for the inositol 1,3,4,5,6-pentakisphosphate 2-kinase (Ipk1) that generates inositol hexakisphosphate. In zebrafish embryos, reducing Ipk1 levels inhibited ciliary beating in Kupffer's vesicle and decreased ciliary length in the spinal canal, pronephric ducts, and Kupffer's vesicle. Electron microscopy showed that ciliary axonemal structures were not grossly altered. However, coincident knockdown of Ipk1 and IFT88 or IFT57 had synergistic perturbations. With GFP-Ipk1 enriched in centrosomes and basal bodies, we propose that Ipk1 plays a previously uncharacterized role in ciliary function.

MeSH Terms (15)

Animals Animals, Genetically Modified Biological Transport Body Patterning Cell Line Centrosome Cilia Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Humans Microtubules Phosphotransferases (Alcohol Group Acceptor) Protozoan Proteins Zebrafish Zebrafish Proteins

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