Detection of ubiquitylated proteins in mammalian cells.

Tansey WP
CSH Protoc. 2006 2006 (6)

PMID: 22485991 · DOI:10.1101/pdb.prot4616

This assay is performed to detect ubiquitylated proteins in transfected mammalian cells. Twenty-four to 40 hours after transfection, cells are harvested, total ubiquitylated proteins are recovered by nickel-affinity chromatography, and specific proteins are detected by Western blotting.

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